Multiplex PCR for Rapid Detection of Genes Multiplex PCR for Rapid Detection of Genes Encoding Class A Carbapenemases
نویسندگان
چکیده
منابع مشابه
Multiplex PCR for Rapid Detection of Genes Encoding Class A Carbapenemases
In recent years, there have been increasing reports of KPC-producing Klebsiella pneumoniae in Korea. The modified Hodge test can be used as a phenotypic screening test for class A carbapenamase (CAC)-producing clinical isolates; however, it does not distinguish between carbapenemase types. The confirmation of type of CAC is important to ensure optimal therapy and to prevent transmission. This s...
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OBJECTIVES To develop a single multiplex real-time PCR assay to detect six different genetic types of carbapenemases already identified in Enterobacteriaceae (KPC, GES, NDM, IMP, VIM and OXA-48). METHODS A total of 58 bacterial isolates were tested. Thirty were previously characterized as resistant to carbapenems and documented by PCR and sequencing analysis to carry the following genes: bla(...
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ژورنال
عنوان ژورنال: Annals of Laboratory Medicine
سال: 2012
ISSN: 2234-3806,2234-3814
DOI: 10.3343/alm.2012.32.5.359